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Image Search Results
Journal: bioRxiv
Article Title: What comes after de novo ? Automated lead optimization of proteins with CRADLE-1
doi: 10.64898/2026.03.06.710001
Figure Lengend Snippet: Comparative Pareto frontier analysis for multi-objective scFv optimization. Successive optimization rounds for an anti-Her2 scFv are visualized across three methodologies: cradle-1 (left), an open-source ESM-2-based method (middle), and alanine scan+CDR-DMS with stacking (right). Plots illustrate the trade-off between expression yield ( E. coli ) and thermal stability ( T m ). Successive cradle-1 rounds demonstrate a clear shift toward the high-performance upper-right quadrant.
Article Snippet: Per reaction mix, 5 μL was transformed into either SHuffle® Express Competent E. coli (NEB, C3028) (for anti-SARS VHH and anti-snake venom VHH) or
Techniques: Expressing