Journal: Microorganisms

Article Title: Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription

doi: 10.3390/microorganisms14030564

Figure Lengend Snippet: Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in E. coli BL21(DE3), and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.

Article Snippet: BL21(DE3) Competent E. coli , C2527H , NEB Inc..

Techniques: Expressing, SDS Page, Plasmid Preparation, Control, Derivative Assay, SDS-Gel

Journal: bioRxiv

Article Title: What comes after de novo ? Automated lead optimization of proteins with CRADLE-1

doi: 10.64898/2026.03.06.710001

Figure Lengend Snippet: Comparative Pareto frontier analysis for multi-objective scFv optimization. Successive optimization rounds for an anti-Her2 scFv are visualized across three methodologies: cradle-1 (left), an open-source ESM-2-based method (middle), and alanine scan+CDR-DMS with stacking (right). Plots illustrate the trade-off between expression yield ( E. coli ) and thermal stability ( T m ). Successive cradle-1 rounds demonstrate a clear shift toward the high-performance upper-right quadrant.

Article Snippet: Per reaction mix, 5 μL was transformed into either SHuffle® Express Competent E. coli (NEB, C3028) (for anti-SARS VHH and anti-snake venom VHH) or BL21 Competent E. coli (NEB, C2530H) (for haloalkane dehalogenase and serine-pyruvate aminotransferase libraries), following NEB’s instructions.

Techniques: Expressing